AtpB | Beta subunit of ATP synthase (rice)

320 €
AS16 3834 | clonality: polyclonal | host: rabbit | ractivity: Oryza sativa


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Item No:
AS16 3834

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product information
Background ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Immunogen KLH-conjugated synthetic peptide chosen from chloroplastic Oryza sativa ATP beta protein sequence UniProt:Q8S6F3; LOC_Os10g21266.1
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100µl of sterile water.
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles.
Tested applications Western blot (WB)
Related products collection of antibodies to various ATPases
Additional information
application information
Recommended dilution 1: 1000 (WB)
Expected | apparent MW

54 kDa

Confirmed reactivity

Oryza sativa

Predicted reactivity Oryza sativa
Not reactive in
Additional information
Selected references To be added when available, antibody released in April 2016.

Application information

western blot using anti-rice ATPase subunit B antibodies

50-100 µg of total protein from Oryza sativa flag leaf, roots or fringe extracted with protein extraction reagent were separated on 15% SDS-PAGE and blotted 1h to PVDF using wet transfer. Blots were blocked with 1×TBST+5% Nonfat dry milk for 1h at 37°C with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 in 1×TBST+5% Nonfat dry milk for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min. and 3 times for 5 min. in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP/conjugated) diluted to 1:15 000 in 1x TBST + 5% Nonfat dry milk for 2h at RT with agitation. The blot was washed as above and developed for 5 min. with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

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