WRKY6 | WRKY transcription factor 6

336 €

AS11 1778 | clonality: polyclonal | host: rabbit | predicted reactivity: Arabidopsis thaliana

product information

background

AtWRKY6 is a transcription factor involved in the control of processes related to senescence and pathogen defense. This protein is interacting with the W box (5'-(T)TGAC[CT]-3'). Alternative names: WRKY transcription factor 6, WRKY DNA-binding protein 6.

immunogen

recombinant part of Arabidopsis thaliana WRKY transcription factor 6 Q9C519, locus At1g62300

antibody format

rabbit

polyclonal

affinity purified serum

quantity

100 µg at 0,33 µg/µl

storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

antibodies to DNA/RNA/cell cycle

additional information

antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth

application information
recommended dilution		1 : 1000 with standard ECL (WB)
expected \| apparent MW		60.5 kDa
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		Brassica napus, Coffea sp. , Pisum sativum
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in March 2012

application example

50 µg of total protein from Arabidopsis thaliana wilde type roots (WT) and wrky6 knock out mutant were separated on 4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 1% BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 80 seconds.

Courtesy Dr. Tzyy-Jen Chiou, Academia Sinica, Taiwan

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