Rnr1 | Ribonucleoside-diphosphate reductase large subunit

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AS16 3639 |  clonality: polyclonal  |  host: rabbit  |  reactivity: Saccharomyces cerevisiae


19 st
Item No:
AS16 3639

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product information

Saccharomyces cerevisiae Rnr1 (EC= is an enzyme from ribonucleoside diphosphate reductase  large chain family. Is localized to cytoplasm and provides precursors necessary for DNA synthesis. Alternative names: ribonucleotide reductase large subunit 1, ribonucleotide reductase R1 subunit 1


KLH-conjugated synthetic peptide derived from Saccharomyces cerevisiae Rnr1 protein sequence P21524

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl

For reconstitution add 50 ĩl of sterile water.


Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS09 574 | anti-Rnr3 antibodies

AS09 575 | anti-Rnr2 antibodies

AS10 847 | anti-Sml1 | Suppressor of Mec1 lethality

Secondary antibodies

Additional information
application information
Recommended dilution

1: 5  000 - 1: 10 000 with ECL (WB)

Expected | apparent MW

99.56 | 100 kDa

Confirmed reactivity

Saccharomyces cerevisiae

Predicted reactivity

Saccharomyces cerevisiae

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment.

Additional information
Selected references To be added when available, antibody released in March 2016. 

application example

western blot using anti-Rnr1 antibodies

10 ul of total protein from a 1 x 108 cells/ml culture of Saccharomyces cerevisiae extracted with 20% TCA and re-suspended in 1X loading buffer (4X SDS loading buffer: 150 mM TrisHCl pH 7.0, 25% Glycerol, 12% SDS, 0.02% Bromophenol Blue, 5% B-mercaptoethanol) were separated on 7.5% SDS-PAGE and blotted to nitrocellulose membrane for 2h/60V using tank transfer. Blots were blocked (5% milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:10000 or 1:5000 o/n at 4°C. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:50 000 or 1: 75 000 in 5% milk for 1h at RT with agitation. The blot was washed as above and developed with Western Lightning ECL Pro (PerkinElmer). Exposure time was between 30 seconds and 1 minute depending on the dilutions.

Courtesy of Dr. Isaac Corcoles, NWCR, United Kingdom

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