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BAM1 | Beta amylase 1 (chloroplastic)

265 €
Buy 2 items of this product for 198 €/each
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AS15 2895  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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Item No:
AS15 2895

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product information
Background Beta amylase (EC 3.2.1.2) is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.
Immunogen The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920., lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.  
Host Rabbit
Clonality Polyclonal
Clone
Purity

Serum

Format

Lyophilized

Quantity

50 µl

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

western blot (WB)

Related products AS15 2896 | anti-BAM3 | Beta amylase 3, rabbit antibodies
antibodies to proteins involved in carbohydrate metabolism
Additional information
application information
Recommended dilution

1:7500 with ECL (WB)

Expected | apparent MW

63.7 | 60.9  kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Beta vulgaris, Brassica napus, Brassica oleracea, Brassica rapa. , Camellia sinensis, Cajanus cajan, Capsella rubella,  Citrus clementina, Citrus sinensis, Coffea canephora, Cucumis sativus, Cynara cardunculus var. scolymus, Daucus carota subsp. sativus, Eucalyptus grandis, Eutrema salsugineum, Glycine max, Gossypium arboreum, Jatropha curcas, Nicotiana tabacum, Phaseolus vulgaris, Poncirus trifoliata, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Theobroma cacao, Vitis vinifera
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Antibody is recognizing recombinant BAM1 protein of Arabidopsis thaliana

Selected references To be added when available, antibody released in September 2016.

application example

western blot using anti-beta amylase 1 antibodies

Arabidopsis thaliana leaf extract (5 ug total protein), BAM1 KO plant (1) and BAM3 KO which still has BAM1 protein (2). Extraction buffer (50 mM MOPS pH 7.0, 5 mM EDTA). Added Equal volume of SSB (final concentration: 50 mM Tris-HCl pH 6.8, 2.5% SDS, 15% glycerol, .05% bromophenol blue, 5% beta-mercaptoethanol) and boiled 5 min. Separated on 10% SDS-PAGE and blotted to nitrocellulose, 100V 1hr using tank transfer. 1 hr block 5% NFDM, PBST, RT 1 hr with primary antibody at 1: 7 500 in 5% NFDM, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min 1 hr in secondary antibody at 1: 20 000, PBST, RT Washed with PBST 2x briefly, then 2x for 10 min Substrate WesternSurePremium (LiCor). Expected BAM3 MW ~64 kDa (usually a doublet).

Courtesy of Dr. Amanda Storm, James Madison University, USA

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com