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BrdU | Bromodeoxyuridine

356 €

AS16 3995 | Clonality: Polyclonal | Host: Sheep | Reactivity: Bromodeoxyuridine

PRODUCT INFORMATION IN PDF

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AS16 3995

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product information
Background

Bromodeoxyuridine is a synthetic nucleoside that is an analog of thymidine which can be integrated into the DNA of proliferating cells as a marker to detect proliferating cells in living tissues.

Immunogen Bromodeoxyuridine, conjugated to Helix Pomatia Haemocyanin.
Host Sheep
Clonality Polyclonal
Clone
Purity Purified using ammonium sulfate precipitation.
Format Liquid
Quantity 250 µg
Reconstitution
Storage Store at -20°C.
Tested applications Immunohistochemistry(IHC), Immunoprecipitation (IP), Western Blot(WB)
Related products anti-sheep secondary antibodies
Additional information Provided in PBS, contains 0.08% sodium azide.

The product has been tested in IP against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU).  This antibody shows a 8-fold higher reactivity with 5-MeC versus BrdU, at a concentration of 25 ug/ml.

Please note: Denature the DNA sample first to make the bases accessible to the BrdU antibody.
application information
Recommended dilution 25-100 µg/ml (IHC), 25-100 µg/ml (IP), 25 -100 µg/ml (WB)
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity known at the moment.
Additional information
Selected references To be added when available, antibody released in November 2016.


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