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BSA | Bovine serum albumin

262 €

AS99 002  |  clonality: polyclonad  |  host: rabbit  |  reactivity: bovine, rat

PRODUCT INFORMATION IN PDF

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Item No:
AS99 002

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product information
Background

Albumin is the most abundant protein in the circulatory system. In mammals albumin is synthesized initially as preproalbumin by the liver. After removal of the signal peptide, the resultant proalbumin is further processed by removal of the six-residue propeptide from the new N-terminus. The albumin released into circulation possesses a half-life of 19 days.

Immunogen

purified BSA, whole molecule P02769

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), ELISA (ELISA)
Related products

AS02 023 | BSA hen antibody

IMS02-035-326 | BSA hen antibody

IMS06-035-327 | BSA hen antibody, biotinylated

AS10 1384 | anti-human albumin goat antibody

anti-rabbit secondary antibodies

Additional information

low endotoxin level as determined by Lonza LAL kit and compare to other products

application information
Recommended dilution

1: 5 000 (WB), 1: 5 000 (ELISA)

Expected | apparent MW

67 kDa

Confirmed reactivity bovine, rat
Predicted reactivity

goat, sheep

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

antibody is recognising endogenous rat serum albumin

Selected references

to be added when available


application example

Indirect ELISA: Anti-BSA antibodies (0.1 mg/ml) at dilution of 1:5 000 was found to generate absorbance 1.0 at 650 nm after 10 minutes of incubation with DAKO® TMB One-Step Substrate System at room temperature.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com