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BSA | Bovine serum albumin

243 €

AS99 002  |  clonality: polyclonad  |  host: rabbit  |  reactivity: bovine, rat

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AS99 002

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product information
background  

Albumin is the most abundant protein in the circulatory system. In mammals albumin is synthesized initially as preproalbumin by the liver. After removal of the signal peptide, the resultant proalbumin is further processed by removal of the six-residue propeptide from the new N-terminus. The albumin released into circulation possesses a half-life of 19 days.

immunogen  

purified BSA, whole molecule P02769

antibody format  

rabbit;

polyclonal;

affinity purified serum in PBS pH 7.4;

lyophilized

quantity  

100µg

 - for reconstitution add 100 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

Western blot (WB), ELISA (ELISA)

related products  

AS02 023 | BSA hen antibody

IMS02-035-326 | BSA hen antibody

IMS06-035-327 | BSA hen antibody, biotinylated

AS10 1384 | anti-human albumin goat antibody

additional information  

low endotoxin level as determined by Lonza LAL kit and compare to other products

application information
recommended dilution  

1: 5 000 (WB), 1: 5 000 (ELISA)

expected | apparent MW  

67 kDa

confirmed reactivity  

bovine, rat

predicted reactivity  

goat, sheep

not reactive in  

no confirmed exceptions from predicted reactivity known in the moment

additional information  

antibody is recognising endogenous rat serum albumin

selected references  

to be added when available


application example

Indirect ELISA: Anti-BSA antibodies (0.1 mg/ml) at dilution of 1:5 000 was found to generate absorbance 1.0 at 650 nm after 10 minutes of incubation with DAKO® TMB One-Step Substrate System at room temperature.


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