Cp2 | Cysteine protease

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AS16 3110 | clonality: polyclonal | host: rabbit | reactivity: Carica papaya


30 st
Item No:
AS16 3110

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product information

Cysteine protease Cp2 shows proteolytic activity and is involved in programmed cell death in plant during pathogen infection.


KLH-conjugated peptide derived from cysteine protease sequence of Carica papaya UniProt: H6USN1

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS14 2771 | anti-SAG12 | Senescence-specific cysteine protease SAG12, rabbit antibody

collection of antibodies to chloroplastic proteases

Plant protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1:1000 with ECL (WB)

Expected | apparent MW

51.4 | 40 kDa

Confirmed reactivity

Carica papaya

Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references to be added when available, antibody released in January 2016. 

application example

western blot using anti-C protease 2 antibodies

Total proteins from Carica papaya cultivar Eksotika and MARDI Purple leaves were extracted using pre-cooled extraction buffer containing 50 mM phosphate buffer pH7 and protease inhibitor cocktail. 60 µg of total protein was blotted 1 h to PVDF using semi-dry transfer cell (BioRad, USA). Blots were blocked with 3% milk in PBST for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in 1% BSA/PBST for 3h at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 5 min each in PBS at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:20 000 in 1% BSA/PBST for 2 h at RT with agitation. The blot was washed as above and stained with Amplified Opti-4CN Substrate kit (BioRad, USA).
Apparent MW of Cp2 is 40 kDa.

Courtesy of Suhaina Supian, Malaysian Agricultural Research and Development Institute, Malaysia

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