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Ca2+-ATPase | Calmodulin-stimulated calcium-ATPase

454 €

AS09 486 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

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AS09 486

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product information
Background

Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type).

Immunogen

KLH-conjugated synthetic peptide derived from Brassica oleracea calmodulin-stimulated calcium-ATPase P93067

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA)
Related products

collection of antibodies to tonoplast proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

This protein is of low abundance in plant tissues.

Protocol for tonoplast membrane isolation can be found here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

Expected | apparent MW

111 | 110 kDa (Raphanus sativus)

Confirmed reactivity

Arabidopsis thaliana, Brassica oleracea, Raphanus sativus

Predicted reactivity

dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticum aestivum, trees: Populus balsamifera, moss: Physcomitrella patens

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Manufactured by Operon Biotechnologies.

Selected references

to be added when available 


application example

 

 

1 µg and 10 µg of crude membrane fraction/lane from Raphabus sativa L. Tokinashi-daikon  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase  antibodies (AS09 486, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

 

  western blot using Ca2+ ATPase antibodies

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com