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CAH3 | Carbonic anhydrase

345 €

AS05 073  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

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AS05 073

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product information
Background

CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3–. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration.

Immunogen

Recombinant carbonic anhydrase of Chlamydomonas reinhardtii  A8J4Z8 after cleavage of a fusion protein

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Related products

antibodies to other photosynthetic proteins

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii.
application information
Recommended dilution 1 : 200 (IF), 1 : 2000 (WB)
Expected | apparent MW

33.4 | 29 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity

Chlamydomonas reinhardtii only

Not reactive in

Arabidopsis thaliana, Oryza sativa

Additional information
Selected references Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.
Mitchell et al. (2014). Dynamics of carbon concentrating mechanism induction and protein re-localisation during the dark to light transition in synchronised Chlamydomonas. Plant Physiol. 2014 Aug 8. pii: pp.114.246918.
Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.
Shutova et al. (2008). The photosystem II-associated Cah3 in Chlamydomonas enhances the O2evolution rate by proton removal. EMBO J. 27:782-791.

application example
western blot
 

(1) Chlamydomonas reinhardtii thylakoid preparation, (2) overexoressed carbonic anhydrase, primary antibody used in 1: 2000 dilution. Secondary antibodies anti-rabbit IgG (GE Healthcare). used at 1: 10 000 with standard ECL

western

20 µg/ml of chlorophyll from Chlamydomonas reinhardtii Cah3-less mutant (1), and 15 µg/ml of chlorophyll from Chlamydomonas reinhardtii wild-type (2),extracted with 4x Laemmli were separated on 12 % SDS-PAGE and blotted 2h to nitrocellulose filter using tank transfer. Blots were blocked with 5% milk powder for for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 fover night at 8°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed once for 15 min and 3 times for 20 min each in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in for 1h at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions. Exposure time was 10min. MW standards used, Page ruler, pre-stained from Thermofisher.


Courtesy of Dr. Göran Samuelsson, Umeå Plant Science Centre, Sweden


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