calcineurin/EF-hand motif

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS13 2666  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Hordeum vulgare


18 st
Item No:
AS13 2666

Info: More information Product suggestions Add review
product information

Calcineurine is a calcium-binding protein involved in signal transduction.


KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2500 with standard ECL (WB)

Expected | apparent MW

20-45 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum

Predicted reactivity Brassica napus, Theobroma cacao,
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

antibody is recognizing recombinant calcineurin expressed in E.coli

Selected references

to be added when available, antibody released in December 2014

application information

western blot using anti/calcineurin hand motiff antibody
0,5µg of purified calcineurin with His-tag was separated on 12% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 5% milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:25 000 in TBS-T containing2% milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 300 seconds.

Courtesy of Dr. Lucyna Misztal, Institute of Molecular Biology and Biotechnology, Poznań, Poland

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at