CBP | CREB-binding protein homolog

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AS13 2730  | clonality: polyclonal  |  host: rabbit  |  reactivity: Drosophila melanogaster



39 st
Item No:
AS13 2730

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product information
Background CBP (CREB-binding protein homolog) is a transcriptional coactivator in multiple signal transduction pathways. It might play a role in variety of cellular processes, including differentiation, cellular proliferation, immune response, homeostasis, tumorigenesis, and erganogenesis.

Recombinant CBP part, fused to GST, derived from O01368

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 500 with standard ECL (WB), 3 µg (ChIP)

Expected | apparent MW

331 kDa

Confirmed reactivity

Drosophila melanogaster

Predicted reactivity Drosophila melanogaster
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

to be added when available, antibody released in March 2014.

application example

western blot using anti-Drosophila CBP antibodies
Total nuclear protein from 2x10^6 Drosophila melanogaster cultured cells was separated on 6% SDS-PAGE and blotted to PVDF using tank transfer. The blot was incubated in the primary antibody at a dilution of 1: 500 in 1% BSA, 0.05% Tween-20, 1x PBS for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice with 1x PBS. Blot was incubated in secondary antibody (anti-rabbit IgG AP-conjugated) diluted to 1:10 000 in 1% BSA, 0.05% Tween-20, 1x PBS for 30 min at RT with agitation. The blot was rinsed twice with 1x PBS and equilibrated in detection buffer (100mM NaCl; 10mM Tris-HCl pH9.5; 10mM MgCl2). The blot was incubated in substrate solution: 165ug/ml of BCIP and 330ug/ml of NBT in detection buffer until the signal reached the desired contrast and then rinsed with water and air dried.

Cortesy of Dr. Tatyana Kahn, Umeå University, Sweden

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