AS16 4096 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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1 : 1000 with ECL (WB)
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana|
|Not reactive in||
No confirmed exceptions from predicted reactivity are currently known
To be added when available, antibody available in
µg of total protein from extracted with write exact buffer components ..... and denatured with....at C or 70C for 2-5 min.... were separated on % SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:0 000 in for 1h at RT with agitation. The blot was washed as above and developed for min with EXACT NAME OF REAGENT AND SUPPLIER. Exposure time was seconds.
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