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product information
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| background |
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Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. |
| immunogen |
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The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC. |
| antibody format |
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rabbit |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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200 µl, |
for reconstitution add 200 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase AS09 524 | anti-MnSOD | manganese superoxide dismutase collection of antibodies to plant stress proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 1000 with alkaline phosphatase (WB) |
| expected | apparent MW |
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33.8 | 37 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
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Arabidopsis thaliana, Brassica juncea, Oryza sativa,Zea mays |
| predicted reactivity |
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dicots including: Glycine max, Solanum tuberosum |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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