CD62P | P-selectin, labelled with fluorescein

304 €

IMS09-143-338    clonality: polyclonal    host: hen    reactivity: human


2 st
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product information

Platelets or thrombocytes are the cell fragments circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots. Dysfunction or low levels of platelets predisposes to bleeding, while high levels, although usually asymptomatic, may increase the risk of thrombosis. Platelets are produced in the bone marrow; the progenitor cell for platelets is the megakaryocyte. It is about twelve times larger than an erythrocyte, possesses a lobed nucleus and sheds platelets into the circulation. Alternative protein names include: CD62P; GMP140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; LECAM3; Leukocyte endothelial cell adhesion molecule 3; PADGEM; Platelet activation dependent granule external membrane protein; PSEL; SELP


recombinant human P-selectin P16109

Host Hen
Clonality Polyclonal
Purity Total IgY fraction
Format Liquid in 0.9 % NaCl, 0.1 % sodium azide
Quantity 100 ĩl (0.7 mg/ml)

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), flow cytometry (FC)
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Additional information

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with fluorescein.

application information
Recommended dilution

1: 1000 (WB), Flow cytometry:  Suitable for detection of platelet activation by flow cytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704, Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated by centrifugation at room temperature. 5 mL platelet-rich plasma was added to polystyrene tubes containing 100 mL HEPES-buffer (137 mmol/L NaCl, 2.7 mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albumin and 20 mmol/L HEPES, pH 7.4) and 10 mL FITC labelled chicken antibody. The samples were incubated for 10 minutes at room temperature and were then diluted and fixed with 1000 mL ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/L NaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing steps were used. The samples were analyzed utilising an Epics Profile XL-MCL cytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 platelets was carried out with the XL software (Coulter Electronics)

Expected | apparent MW

91 kDa

Confirmed reactivity

human, porcine

Predicted reactivity


Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

the antibodies have been tested in flow cytometry to provide reproducible results; antibodies will react with activated human and porcine platelets according to the method Larsson A et al. Platelets. 2002 May;13(3):153-7 Studies of fibrinogen binding to porcine platelets by flow cytometry: a method for studies of porcine platelet activation.

Selected references

to be added when available

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