Chicken anti-Rat IgG (H&L) , DyLightŪ 594 conjugated, min. cross-reactivity to human or rabbit IgG or serum proteins

175 €

AS12 2088 | clonality: polyclonal  |  host: chicken   |   reactivity: rat IgG (H&L)


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Item No:
AS12 2088

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product information

Chicken anti-Rat IgG (H&L) , DyLight® 594 Conjugated, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to  DyLight® 594, which binds to rat IgG (H&L) in immunological assays. 

DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rat IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.


Purified rat IgG, whole molecule

Host Chicken
Clonality Polyclonal
Purity Affinity purified chicken IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products

other dylight anti-rat secondary antibodies

Tested applications
Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on rat IgG  light chains on all rat immunoglobulins.

No reactivity is observed to: non-immunoglobulin rat serum proteins  IgG from human or rabbit  serum proteins from human or rabbit.

application information
Recommended dilution

1:20 - 1:2,000 for most applications

Selected references

to be added when available

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