Chicken anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, rabbit IgG/serum

197 €

AS10 1107   | clonality: polyclonal  |  host:chicken   |  reactivity:rat IgG (H&L)


0 st
Item No:
AS10 1107

Info: Product suggestions Add review
product information
Background Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other HRP conjugated secondary antibodies to rat IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

application information
Recommended dilution 1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.
Selected references

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at