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Chicken anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to human, mouse IgG

181 €

AS10 835 | clonality: polyclonal  |  host: chicken  | reactivity: rabbit IgG (H&L)

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AS10 835

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product information
Background

Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.

Immunogen

Purified Rabbit IgG

Host Chicken
Clonality Polyclonal
Clone
Purity Affinity purified Chicken IgY
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

other antibodies to rabbit IgG HRP or alkaline phosphatase (ALP) conjugated

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity rabbit IgG (H&L)
Predicted reactivity

rabbit IgG (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis.

Selected references

to be added when available



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