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Chicken anti-Rat IgG (H&L), FITC conjugated, min. cross-reactivity to human, rabbit IgG/serum

197 €

AS10 1012   | clonality: polyclonal  |  host:chicken   |  reactivity:rat IgG (H&L)

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AS10 1012

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product information
Background Chicken anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Immunogen
Host Chicken
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Related products other fluorescent dye conjugated secondary antibodies to rat IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed to human and rabbit serum or IgG based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution 1:20-1: 2000 for immunohistochemistry (IHC),1:50-1: 5000 for immunocytochemistry (ICC) and for flow cytometry.
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