CHIT3 | Chitinase 3

293 €

AS15 2888 | clonality: polyclonal | host: rabbit | reactivity:


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Item No:
AS15 2888

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product information
Background Chitinase 3 is a protein that hydrolyzes chitin and has a great impact in the defense against fungal pathogens that contains chitin. Alternative names: Basic endochitinase 1, Class I chitinase c, OsChia1c, Pathogenesis related (PR)-3, chitinase 3.

KLH-conjugated synthetic peptide derived from Oryza sativa OsCht3 protein sequence, UniProt:P24626 CHI3_ORYSJ

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage The antibody may be stored at -20℃f or one year in its original formulation. Additionally, antibody may be stored at 2℃ to 8℃ for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.

Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
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AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibody for Arabidopsis thaliana PR-2 protein detection
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AS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodies
AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies

collection of antibodies to other proteins involved in a response to pathogen attack

Secondary antibodies

Additional information
application information
Recommended dilution

1:1000 (WB)

Expected | apparent MW

33.68 kDa

Confirmed reactivity

Predicted reactivity Musa paradisiaca, Zea mays
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references to be added when available, antibody released in June 2015

Application example:

Total protein from Oryza sativa rice (CV. 9311) flag leaf at the flowering stage was ground into a fine powder in liquid nitrogen. An 800 ul aliquot of extraction buffer: 62.5 mM TRIS-HCl (pH 7.4), 10% glycerol, 0.1% SDS, 2 mM EDTA, 1 mM phenylmethylsulphonyl fluoride (PMSF), 5% (v/v) b-mercaptoethanol] was added to each 300 mg powder sample. The mixture was vortexed and then chilled on ice for 10 min. Samples were centrifuged at 12,000 rpm for 10 min. at 4℃, and the supernatant was collected and stored at –70℃. The protein concentrations of the rice samples were determined using the Bradford method (Bradford, 1976). 20 µg of protein was separated on 12 % SDS-PAGE and blotted 1h to PVDF.
Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 30 sec.

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