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CO4 | Complement component C4

304 €

IMS02-032-303  |  clonality: polyclonal  |  host: hen  |  reactivity: human

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IMS02-032-303

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product information
Background

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Immunogen

full length native protein P0C0L4 purified from human plasma

Host Hen
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Quantity 100 ĩl (1 mg/ml)
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB), immunodifusion (ID)
Related products

IMS06-032-304 | anti-Complement component C4, biotinylated

IMS01-031-305 | anti-Complement component C4c

IMS01-066-309 | anti-Complement factor H

Secondary antibodies

Additional information

affinity purified IgY on human C4 agarose

application information
Recommended dilution

1: 2000 (ELISA), 1: 1000 with standard ECL (WB)

Expected | apparent MW

193 kDa

Confirmed reactivity

human

Predicted reactivity

human

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Only one precipitin arc in immunodiffusion against normal human serum or human complement component C4. Only one precipitin arc in immunoelectrophoresis against normal human serum or human complement component C4.

ELISA has been performed on C4 deposition using human and mouse serum.

Selected references

to be added when available



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