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CO4c | Complement component C4c

304 €

IMS01-031-305  |  clonality: polyclonal  |  host: hen  |  reactivity: human

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IMS01-031-305

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product information
Background

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Immunogen

full lenth native protein P0C0L4 purified from human plasma

Host Chicken
Clonality Polyclonal
Clone
Purity Total IgY fraction
Format Liquid in 0.9% NaCl, 0.1% sodium azide
Quantity 100 ĩl (25.9 mg/ml)
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

IMS02-032-303 | anti-Complement component C4

IMS06-032-304 | anti-Complement component C4, biotinylated

IMS01-066-309 | anti-Complement factor H

Secondary antibodies

Additional information

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information
Recommended dilution

use at an assay dependent dilution (WB)

Expected | apparent MW

35 kDa

Confirmed reactivity human
Predicted reactivity

human

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Only one precipitin arcs in immunodiffusion against normal human serum or human C4. Reacts with α and β chain in electroblotting.

Selected references Bidula et al. (2013). The role of ficolin-A and lectin complement pathway in the innate defence against pathogenic Aspergillus species. Infect. Immun. March 11.


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