COXII | Cytochrome oxidase subunit II (200 ĩg)
AS04 053A-200 | clonality: polyclonal | host: rabbit | reactivity: eudicots, monocots, Physcomitrella patens | cellular [compartment marker] of mitochondrial inner membrane
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|Expected | apparent MW||
29.4 | 30 kDa (for Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica|
dicots including Glycine max, Pisum sativum, monocots including Oryza sativa, Zea mays, moss Physcomitrella patens
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
antibody detects COXII protein in total leaf extracts and isolated mitochondria
|Selected references||Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,salicylic acid, and pathogen elicitors. J Exp. Bot. 61:831-841.
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.
1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.
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