COXII | Cytochrome oxidase subunit II (200 ĩg)
AS04 053A-200 | clonality: polyclonal | host: rabbit | reactivity: eudicots, monocots, Physcomitrella patens | cellular [compartment marker] of mitochondrial inner membrane
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|Expected | apparent MW||
29.4 | 30 kDa (for Arabidopsis thaliana)
Arabidopsis thaliana (leaf extract and isolated mitochondria), Brassica oleracea, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica
dicots including Glycine max, Pisum sativum, monocots including Oryza sativa, Zea mays, moss Physcomitrella patens
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
antibody detects COXII protein in total leaf extracts and isolated mitochondria
Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)
Mika et al. (2010). Membrane-bound guaiacol peroxidases from maize (Zea mays L.) roots are regulated by methyl jasmonate,
Leroch et al (2008). Identification of a novel adenine nucelotide transporter in the endoplasmic reticulum of Arabidopsis. The Plant Cell 20:438-451.
1.8 µg of total protein from (1) Arabidopsis thaliana leaf , (2) Plantago major leaf, (3) Plantago europhylla leaf, (4) Silene dioica leaf, (5) Silene uniflora leaf were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Primary antibody was used in 1: 1000 dilution.
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