|
product information
|
| background |
|
Chl27 catalyzes the cyclase reaction in chlorophyll biosynthesis. |
| immunogen |
|
residues 1-409 from Arabidopsis thaliana CHL27 fused to TrxA Q9M591 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
100 µl |
for reconstitution add 100 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane Collection of antibodies to Arabidopsis proteins Collection of antibodies to Chlamydomonas proteins |
| additional information |
|
[compartment marker] of chloroplast thylakoid and envelope membranes |
application information
|
| recommended dilution |
|
1:3000 with alkaline phosphatase (WB) |
| expected | apparent MW |
|
47 | 40 kDa (Arabidopsis thaliana) |
| confirmed reactivity |
|
Arabidosps thaliana, Horderum vulgare, Nicotiana tabacum, Pisum sativum, moss: Physcomitrella patens, Chlamydomonas reinhardtii, purple bacteria (CRD1) and (CHL27) |
| predicted reactivity |
|
eudictos:Gossyium hirsutum; Euphorbia esula; Ricinus communis, monocots Hordeum vulgare, cyanobacteria |
| not reactive in |
|
no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
|
Antibodies detect two isoforms in Chlamydomonas reinhardtii, CRD1 in cells grown under copper deficiency (39.8 kDa) and CTH1 in cells grown with sufficient copper (40.7 kDa). Antibodies will also react with Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, and purple bacteria |
| selected references |
|
Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source). Peter et al. (2010). Mg protoporphyrin monomethylester cyclase deficiency and effects on tetrapyrrole metabolism in different light conditions. Plant Cell Physiol. 51(7):1229-1241. Moseley et al. (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:673-688. Tottey et al. (2003) Arabidopsis CHL27, located in both envelope and thylakoid membranes, is required for the synthesis of protochlorophyllide. Proc. Natl. Acad. Sci. USA 100:16119-16124. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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