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CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C
Product no: AS14 2760
AS14 2760 | Clonality:Polyclonal | Host:Rabbit | Reactivity: proteobacteria
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319
€
Delivery:
3-6 business days
- Product Info
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Immunogen: KLH-conjugated peptide conserved in Major carboxysome shell protein 1A, UniProt: P45689, Major carboxysome shell protein 1B UniProt: P45690, Major carboxysome shell protein 1C, UniProt:
P45688Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 9-11 kDa - Reactivity
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Confirmed reactivity: Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus) Predicted reactivity: Algae (red), Cyanobacteria, Cryptomonads
Species of your interest not listed? Contact usNot reactive in: Synechococcus elongatus - Application Examples
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application example 
0.125 - 1.0 µg of total purified Halothiobacillus neapolitanus carboxysome protein (equivalent to approximately 20 - 170 ng CsoS1A, B and C proteins) were separated on 4-20 % Bio-Rad TGX stain-free SDS-PAGE and blotted 1h to PVDF using a Bio-Rad TransBlot Turbo Blotting System. Blots were blocked with 5% skim milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat-anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:5 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and antibody binding determined using the Promega Attophos reagent system as described by the manufacturer. Reaction time was 10 seconds and membranes were imaged using a Bio-Rad VersaDoc.
Courtesy of Dr. Ben Long, The Australian National University, Australia - Background
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Background: Major carboxysome shell protein is involved in the formation of the carboxysome, polyhedral includion sequestering Rubisco. - Product Citations
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Selected references: Huang, Jiang, Yang, et al. (2022) Probing the Internal pH and Permeability of a Carboxysome Shell. Biomacromolecules. 2022;23(10):4339-4348. doi:10.1021/acs.biomac.2c00781
Chen et al. (2022) ACS Synth. Biol. 2022, 11, 1, 154-161Publication Date:October 19, 2021 https://doi.org/10.1021/acssynbio.1c00311
Li et al. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020 Oct 28;11(1):5448. doi: 10.1038/s41467-020-19280-0. PMID: 33116131; PMCID: PMC7595155.
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
- Reviews:
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Ben | 2016-05-01This antibody is highly specific to csoS1 proteins with great detection limit when used in western blots on purified carboxysomes from Halothiobacullus neapolitanus. We have also had success in detecting csoS1 proteins from cyanobacterial sources both in the native host and expressed in heterologous systems.
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items