CSP41b | ribosome associated endonuclease (CRB)

345 €

AS08 298 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Pisum sativum, Physcomitrella patens


18 st
Item No:
AS08 298

Info: Product suggestions Add review
product information

CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.


KLH-conjugated synthetic peptide derived from CSP41b protein sequence of Arabidopsis thaliana Q9SA52, At1g09340

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to various chloroplastic proteins

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

42 | 39 kDa

Confirmed reactivity

Arabidopsis thaliana, Pisum sativum, Physcomitrella patens

Predicted reactivity

dicots including: Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchenis, Populus sp.,

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).

Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.

Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.

application information

10 µg of total leaf protein from P.sativum (1) was separated on 4-12% NuPage (Invitrogen) and blotted on nitrocellulose. Molecular marker was MagicMark (Invitrogen). Filters where blocked overnight in TBST containing 5 % milkpowder and incubated with 1:1000 anti-CSP41b antibody (AS08 298, 1h). After incubation with 1:10000 secondary HRP coupled anti-rabbit antibody (1h) signals were visualized with standard ECL (GE Healthcare).

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at