CSP41b | ribosome associated endonuclease (CRB)
AS08 298 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
42 | 39 kDa
Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
dicots including: Vitis vinifera, monocots including: Oryza sativa, Zea mays, trees: Picea sitchenis, Populus sp.,
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.
Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
10 µg of total leaf protein from P.sativum (1) was separated on 4-12% NuPage (Invitrogen) and blotted on nitrocellulose. Molecular marker was MagicMark (Invitrogen). Filters where blocked overnight in TBST containing 5 % milkpowder and incubated with 1:1000 anti-CSP41b antibody (AS08 298, 1h). After incubation with 1:10000 secondary HRP coupled anti-rabbit antibody (1h) signals were visualized with standard ECL (GE Healthcare).
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