CSP41b | ribosome associated endonuclease (CRB)
AS08 298 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
42 | 39 kDa
|Confirmed reactivity||Arabidopsis thaliana, Pisum sativum, Physcomitrella patens|
|Predicted reactivity||Oryza sativa, Picea sitcHenis, Populus sp., Zea mays, Vitis vinifera|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.
Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
10 µg of total leaf protein from P.sativum (1) was separated on 4-12% NuPage (Invitrogen) and blotted on nitrocellulose. Molecular marker was MagicMark (Invitrogen). Filters where blocked overnight in TBST containing 5 % milkpowder and incubated with 1:1000 anti-CSP41b antibody (AS08 298, 1h). After incubation with 1:10000 secondary HRP coupled anti-rabbit antibody (1h) signals were visualized with standard ECL (GE Healthcare).
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