Donkey anti-Mouse IgG (H&L), Affinity Pure, DyLightŪ 650 conjugated,min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

175 €

AS12 2035 | clonality: polyclonal  |  host: donkey  | reactivity: mouse IgG


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Item No:
AS12 2035

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product information

Donkey anti-Mouse IgG (H&L) - Affinity Pure, DyLight®650 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight® 650, which binds to all anti-mouse IgG in immunological assays. Amax = 593 nm, Emax = 618 nm.

Antibodies are are affinity purified using solid phase mouse IgG.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.


Purified mouse IgG

Host Donkey
Clonality Polyclonal
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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other dylight anti-rabbit secondary antibodies

Tested applications
Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.

Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins  IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat.


western blot using Agrisera donkey anti-mouse IgG antibodies
Donkey anti-mouse conjugated to DY Light 650 in the absence of primary antibody show very little background label (left images) and binds to primary antibody (anti-α-tubulin; right images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Mouse anti-α-tubulin DM1A (Santa Cruz, Sc-32293) was diluted 1:200 and Donkey anti-mouse DY Light 650 was diluted 1:100. DNA was stained with DAPI.

Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Umeå Plant Science Centre
application information
Recommended dilution

1:20 - 1:2 000 for most applications

Selected references

to be added when available

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