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Donkey anti-Rabbit IgG (H&L), ALP conjugated

181 €

AS10 841 | clonality: polyclonal  |  host: donkey  | reactivity: rabbit IgG (heavy and light chains - H&L)

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AS10 841

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product information
Background

Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG  (H&L) in immunological assays.

Immunogen

Purified Rabbit IgG

Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified donkey IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC)
Related products

AS09 607 | Goat anti-rabbit IgG (H&L) ALP (alkaline phosphatase) conjugated

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

application information
Recommended dilution

1: 500 - 2 000 (ELISA) and  (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Expected | apparent MW
Confirmed reactivity

rabbit IgG (H&L)

Predicted reactivity

rabbit IgG (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Selected references Kay et al. (2014). Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.


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