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Donkey anti-Rabbit IgG (H&L), ALP conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG

203 €

AS10 1127 | clonality: polyclonal | host: donkey | reactivity:rabbit IgG (H&L)

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AS10 1127

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product information
Background Donkey anti-rabbit IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
Immunogen
Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 1 mg
Reconstitution
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Related products other ALP conjugated secondary antibodies to rabbit IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG, based on immunoelectrophoresis.

Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.

application information
Recommended dilution 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
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