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Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLightŪ 488 conjugated

203 €
AS16 3526 | Clonality: Polyclonal | Host: Donkey | Reactivity: Rabbit IgG (H&L), F(ab)'2 Fragment

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AS16 3526

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product information
Background

Donkey anti-Rabbit IgG (H&L), F(ab)'2 Fragment, DyLight® 488 conjugated is a secondary antibody conjugated to  DyLight® 488, which binds to rabbit IgG in immunological assays. 
DyLight® 488 has Amax = 493 nm, Emax = 518 nm.

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Immunogen Purified Rabbit IgG, whole molecule
Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution add 0.55 ml of sterile water.

Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Storage Store freeze-dried powder at 2-8 °C.
Tested applications Flow cytometry (FACS), Immunomicroscopy (IM)
Related products Other anti-rabbit secondary antibodies
Additional information

Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Rabbit IgG .
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on rabbit IgG • light chains on all rabbit immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins .

application information
Recommended dilution

1:20 - 1:2 000 (FACS and IM)

Expected | apparent MW
Confirmed reactivity

Rabbit IgG (H&L)

Predicted reactivity
Not reactive in
Additional information
Selected references To be added when available, antibody released in April 2016.


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