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Donkey anti-Rat IgG (H&L), F(ab)'2 fragment, DyLightŪ 800 conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, sheep IgG

238 €

AS12 2470 | clonality: polyclonal | host: donkey | reactivity: rat IgG (H&L), F(ab)'2 fragment

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AS12 2470

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product information
Background

Donkey anti-Rat IgG (H&L), F(ab)'2 fragment, DyLight®800 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rabbit or sheep IgG is a secondary antibody conjugated to DyLight® 800, which binds to Rat IgG (H&L), F(ab)'2 fragment in immunological assays.

DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Rat IgG (H&L)

DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Immunogen

Purified Rat IgG, whole molecule

Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified donkey IgG
Format Lyophilized
Quantity 0.5 mg
Reconstitution For reconstitution please add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications
Related products

other DyLight® anti-Rat secondary antibodies

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulins

No reactivity is observed to: non-immunoglobulin rat serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep

application information
Recommended dilution

1:20 - 1:2,000 for most applications

Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references

to be added when available



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