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Donkey anti-Sheep IgG (H&L), FITC conjugated

203 €

AS10 1171   | clonality: polyclonal  |  host:donkey   |  reactivity:sheep IgG (H&L)

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AS10 1171

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product information
Background Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase sheep IgG.
Immunogen
Host Donkey
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 2 mg
Reconstitution
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Related products other fluorescent dye conjugated secondary antibodies to sheep IgG
Tested applications
Additional information

This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.

BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
Selected references


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