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Donkey normal serum (10 ml)

168 €
AS10 1564 | Host: Donkey


For larger volumes, please inquire


PRODUCT INFORMATION IN PDF

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Item No:
AS10 1564

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product information
Background Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Host Donkey
Clonality
Clone
Purity Serum
Format Lyophilized
Quantity 10 ml
Reconstitution For reconstitution add 11.0 ml of sterile water.
Storage Store lyophilized material at 2-8 ºC. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC . For storage at 2-8 ºC, add a preservative to prevent growth of bacteria. Rehydrate with 10.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Tested applications
Related products

other blocking reagents derived from donkey

secondary antibodies

Additional information

Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.


Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
application information
Recommended dilution
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com