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Donkey normal serum (2 ml)

122 €
AS10 1563 | host: donkey


For larger volumes, please inquire


PRODUCT INFORMATION IN PDF

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AS10 1563

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product information
Background Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Host Donkey
Clonality
Clone
Purity Serum
Format Lyophilized
Quantity 2 ml
Reconstitution
Storage Store lyophilized material at 2-8 ºC. For long term storage after reconstitution, prepare small aliquots and store at -20 ºC . For storage at 2-8 ºC, add a preservative to prevent growth of bacteria. Rehydrate with 2.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Tested applications
Related products

other blocking reagents derived from donkey serum

secondary antibodies

Additional information

Recommended dilution: 2-5% (v/v) serum in PBS/TBS.  To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.

For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.

Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.

application information
Recommended dilution
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information
Selected references


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