DRB4 | Double-stranded RNA-binding protein 4

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AS15 3104 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


30 st
Item No:
AS15 3104

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product information
Double-stranded RNA-binding protein 4 (DRB4) plays a role in RNA-mediated post-transcriptional gene silencing (PTGS). It assists DCL4 during biogenesis of trans-acting small interferring RNAs (ta-siRNAs) and is necessary for DCL4 activity. DRB4 is also involved in RNA silencing of RNA and DNA viruses.

Alternative name: dsRNA-binding protein 4, DBR4
Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana DRB4 sequence, Uniprot: Q8H1D4, TAIR: At3g62800
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western Blot (WB)
Related products

anti-SGS3 | Protein suppressor of gene silencing 3 antibody
anti-DCL1 | Dicer-like protein 1 antibody
anti-DCL2 | Dicer-like protein 2 antibody
anti-DCL3 | Dicer-like protein 3 antibody
anti-DCL4 | Dicer-like protein 4 antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000

Expected | apparent MW

38.4 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica napus, Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum
Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Additional information

To be added when available.

Selected references

To be added when available, antibody released in October 2016.

 Application example
Western Blot with anti-DRB4 antibody

100 µg of total protein from Arabidopsis wt, drb4, and 35S-DRB4  leaves extracted with isolation buffer (50 mM Tris-HCl, pH 7.5, 10% glycerol, 150 mM NaCl, 10 mM MgCl2, 5 mM EDTA, 5 mM DTT, and 1 X protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and denatured by boiling at 100°C. The proteins were separated on 10% SDS-PAGE and blotted 1.5 hrs to PVDF membrane using wet transfer. Blots were blocked with 5% milk powder  for 30 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1,000 for 60 min at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in phosphate buffer (containing 0.1% Tween 20) at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:10,000 for 120 min at RT with agitation. The blot was washed as above and developed with  NBT/BCIP (Fisher Chemicals) using Alkaline phosphatase detection system. The DRB4 specific band was seen within 20 mins and showed a better signal when allowed to develop for longer duration (overnight in this case).

Courtesy of Dr. Gah-hyun Lim and Prof. Pradeep Kachroo, University of Kentucky, Lexington, USA

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