Dsp | death-specific protein

339 €

AS10 935 | clonality: polyclonal | host: rabbit | reactivity: diatoms



15 st
Item No:
AS10 935

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product information

KLH-conjugated synthetic peptide derived from known sequences of DSP in diatoms, conserved in Dsp1 and Dsp2, UniProt: B6RD07  of Thalassiosira pseudonana and Skeletonema costatum and Dsp1 in Fragilariopsis cylindrus and Phaeodactylum tricornutum

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 10 000 with standard ECL (WB)

Expected | apparent MW

25 | 25 kDa

Confirmed reactivity

Emiliania huxleyi, Aureococcus anophagefferens, Fragilariopsis cylindrus, Phaeodactylum tricornutum, Skeletonema costatum, Thalassiosira pseudonana

Predicted reactivity
Not reactive in

Aureococcus anophagefferens

Additional information
Selected references

Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. PNAS 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Epub 2013 Nov 25.

application example

western blot using anti-DSP antibodies

5 µg of total protein, extracted with LDS sample buffer (PSB) were separated on 4-20% SDS-PAGE and transferred to PVDF. Blot was blocked with 2% ECL block in TBS-T (0.1% Tween-20) for 1h at room temperature (RT) with agitation. Blot was incubated in primary antibody at a dilution of 1: 10,000 for 1h at RT with agitation. Antibody solution was decanted and the blot rinsed briefly twice with TBS-T, then washed once for 15 min and 3 times for 5 min. at RT with agitation. Blot was incubated in secondary antibody (horse radish peroxidase conjugated anti-rabbit IgG) at a dilution of 1:20 000, for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Image (20 sec. exposure) captured with a BioRad Chemidoc MP.
Predicted activity and molecular weights (Da) - Thalassiosira pseudonana Dsp1 (24572 Da) Dsp2 (25406 Da), Skeletonema costatum Dsp1 (24848 Da) Dsp2 (26566 Da), Phaeodactylum tricornutum Dsp1 (26585 Da, Fragilariopsis cylindrus Dsp1 (20312 Da).

Competition assay (right half of blot): Same protein loads as for lanes 1-7. Antisera were pre-incubated for 90 min. in TBS-T with of an excess of the free peptide antigen

Courtesy of Dr. K. Thamatrakoln unpublished data; rights retained by the contributor.

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