EDS1 | Enhanced disease susceptibility 1

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AS13 2751 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


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AS13 2751

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product information

EDS1 (Enhanced disease susceptibility 1) is a protein with lipase activity involved in aerencyma formation, lipid metabolic process, response to hypoxia and systemic acquired resistance. Alternative names: Putative disease resistance protein EDS1, Putative uncharacterized protein T17F15.40.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana EDS1 sequence, UniProt: Q9SU72, TAIR: AT3G48090

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to pathogen attack
collection of antibodies to hypoxia

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 3000 with standard ECL (WB)

Expected | apparent MW

71.6 kDa | 72 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Arabidopsis thaliana
Not reactive in

Nicotiana benthamiana

Additional information

to be added when available

Selected references

to be added when available, antibody released in September 2014.

application example

western blot using anti-EDS1 antibodies

20 µg of total protein from Arabidopsis thaliana extracted with HEPES buffer were separated on 8% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5% milk-TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 minutes.

Courtesy of Morgan K. Halane, University of Missouri, USA

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