eEF1a | Elongation factor 1-alpha

345 €

AS10 934  | clonality: polyclonal  |  host: rabbit  |  reactivity:A. thaliana, H.vulgare, Z.mays, O. europaea, P.patens


8 st
Item No:
AS10 934

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product information

EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.


KLH-conjugated synthetic peptide derived from known sequences of eEF1a including Arabidopsis thaliana EF-1-alpha 1P0DH99 AT1G07940  EF-1-alpha 2 Q8W4H7  AT1G07930, EF-1-alpha 3 Q0WL5 AT1G07920, EF-1-alpha 4 Q8GTY0 AT5G6039

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha - antibody made to a recombinant EF1A from Arabidopsis thaliana

AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS07 265 | eEF1b | elongation factor eEF1b-beta protein

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

to be added when available

application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

49.5 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Zea mays, Olea europaea, Physcomitrella patens

Predicted reactivity dicots including: Daucus carota, Glycine max, Nicotiana tabacum, Pisum sativum, Solanum lycopersicum, Vicia faba, monocots including: Oryza sativa, Triticum aestivum, trees: Malus domestica, other species: Lilium longiflorum, unicellular flagellate protists: Euglena gracilis
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available, antibody released in November 2012.

application example

western blot using anti-plant elongation factor antibody eEF1a

5 µg of total protein from Arabidopsis thaliana (A), Hordeum vulgare (B), Zea mays (C), Physcomitrella patens (P)  extracted with Agrisera PEB buffer  were separated on 4-12 % SDS-PAGE and blotted 70 min to PVDF. Blots were blocked with ECL Advance Blocking Reagent (GE Healthcare)  for 1h at room temperature (RT) with agitation. Blot was incubated for 1 h with either rabbit pre-serum (1: 10 000), rabbit anti-eEF1alpha antibody (1: 10 000) and anti-eEF1 alpha serum incubated with eEF1alpha synthetic peptide (in 100 mM excess to saturate a signal from specific antibody) for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera  ) diluted to 1:50 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was  2 minutes.

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