eEF1B-alpha2 | elongation factor 1B-alpha 2

345 €

AS10 679 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana


15 st
Item No:
AS10 679

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product information

eEF1B-alpha2 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-beta 2, Elongation factor 1-beta' 2, EF-1-beta' 2


Recombinant eEF1B-alpha2 protein from Arabidopsis thaliana with no affinity tag, Q9SCX3, At5g19510

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS07 265 | eEF1b | elongation factor eEF1b-beta protein

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2collection of antibodies to DNA/RNA metabolism

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibody shows a very weak cross-reaction to elongation factor 1B-alpha 1.

application information
Recommended dilution

1: 2000 with standard ECL (WB)

Expected | apparent MW

24 | 34 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

dicots including: Glycine max, Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, Zea mays

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available

application example
western blot detection using anti-alpha2 antibodies

10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Amersham diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

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