eEF1b | Elongation factor 1-beta

345 €

AS07 265 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays


5 st
Item No:
AS07 265

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product information

eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2


Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, Q9SI20, At2g18110

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha

AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2

collection of antibodies to DNA/RNA metabolism

Additional information

to be added when available

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

25 | 30 kDa

Confirmed reactivity

Arabidopsis thaliana, Zea mays

Predicted reactivity

dicots including: Brassica campestris. Pisum sativum, Ricinus communis, monocots including: Hordeum vulgare, Oryza sativa

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.

application example


10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).

western blot using anti-eEF1b antibodies

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