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eEF1b | Elongation factor 1-beta

345 €

AS07 265 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays

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Item No:
AS07 265

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product information
Background

eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2

Immunogen

Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, Q9SI20, At2g18110

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha

AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2

collection of antibodies to DNA/RNA metabolism

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

25 | 30 kDa

Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Brassica campestris, Hordeum vulgare, Oryza sativa Pisum sativum, Ricinus communis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.

application example

 

10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000.



western blot using anti-eEF1b antibodies

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