eEF1b | Elongation factor 1-beta
AS07 265 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
25 | 30 kDa
Arabidopsis thaliana, Zea mays
dicots including: Brassica campestris. Pisum sativum, Ricinus communis, monocots including: Hordeum vulgare, Oryza sativa
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
to be added when available
Lázaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.
10 µg of total cell extract from Arabidopsis thaliana (1), 5 µg of total cell extract from Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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