EF-G1 | elongation factor G1

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AS13 2652 | clonality: polyclonal | host: rabbit | reactivity: Synechocystis sp. PCC6803


26 st
Item No:
AS13 2652

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product information

EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.


Recombinant EF-G1 protein from Synechocystis sp. PCC6803, P28371, Cyanobase: slr1463

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
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AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and collection of antibodies to DNA/RNA metabolism

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 4000 with standard ECL (WB)

Expected | apparent MW

76.7 kDa

Confirmed reactivity

Synechocystis sp. PCC6803

Predicted reactivity


Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

to be added when available, antibody released in November 2013.

application example

western blot using anti-EF-G1 antibodies

Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T  for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was  2 minutes.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

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