EF1A | Elongation factor 1-alpha / EF-1-alpha

345 €

AS11 1633   | clonality: polyclonal  |  host: rabbit  |  reactivity:Arabidopsis thaliana


26 st
Item No:
AS11 1633

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product information

EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.


full length, recombinant EF1A of Arabidopsis thaliana P13905

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS07 265 | eEF1b | elongation factor eEF1b-beta protein

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

49.5 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity dicots including: Brassica napus, Solanum tuberosum, Sorghum bicolor,monocots including: Oryza sativa, Zea mays, trees: Populus trichocarpa, Picea sitchensis
Not reactive in

recombinant wheat EF1A

Additional information

to be added when available

Selected references

to be added when available. Antibody released in February 2011.

application example

western blot detection of plant EF1A
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22°C (3) or heat treated at 38°C for 2 hours  prior to protein extraction (4). As positive control 10 ng of recombinant AtEF1A (2) were separated side by side with the plant samples on 10-15 % gradient SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1000 for O/N 4°C with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody, anti-rabbit IgG horse radish peroxides conjugated, from Agrisera, AS09 602, diluted to 1:35 000 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturer's instructions.  Exposure time was 5 seconds.

Lane (1) with no signal: recombinant Triticum spp. EF1A.

Courtesy Dr. Eman Basha, Arizona State University

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