EIN2 | Ethylene insensitive 2

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AS12 1865 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


16 st
Item No:
AS12 1865

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product information

EIN2 (Ethylene-insensitive protein 2) is a central factor in signaling pathways regulated by ethylene including various processes like: plant defense and development, senescence, nucleotide sugar flux and tropisms. Alternative names: EIN2, ETHYLENE INSENSITIVE 2, PIR2, CKR1, CYTOKININ RESISTANT 1, ERA3, ENHANCED RESPONSE TO ABA3, ORE3, ORESARA 3, ORE2, ORESARA 2, ATEIN2.

Immunogen KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana, UniProt: Q9S814, TAIR: AT5G03280
Chosen peptide is not to be found in EIN3.
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2866 | anti-EIN3 | Ethylene insensitive 3, rabbit antibody
AS06 193 | anti-IAA | indole 3 acetic acid, rabbit antibody
AS11 1749 | IAA | auxin ELISA quantitation kit

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 2000 (WB)
Expected | apparent MW

140 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brachypodium distachyon, Glycine max, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Phtheirospermum japonicum, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Triticum aestivum, , Zea mays, Vitis vinifera (Please inquire for other species)
Not reactive in no confirmed exceptions from predicted reactivity known in the moment
Additional information
Selected references to be added when available, antibody released in April 2015

application example

western blot using anti-EIN2 antibodies

40-60 µg of membrane protein (measured with Millipore Direct Detect spec.) isolated following membrane extraction buffer protocol from Dong et al. 2008 (Plant Journal 53(2): 275-286) from 4 day old dark grown Arabidopsis thaliana seedlings, grown on MS plated with ACC or AVG in the plate, extracted with (10 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 10 % glycerol, 1 % Triton X-100, and protease ihibitors cocktail from Sigma) were separated 4-20% Bio-Rad precast SDS-PAGE gel and blotted 1h to PVDF membrane using semi-dry transfer. Blots were blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was then washed 5 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:20 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Bio-Rad ECL according to the manufacturer's instructions. Exposure time was 25 min (to see full length EIN2).

Antibody is suitable for detection of full length EIN2. Bands below 140 kDa are non-specific.

Courtesy of Jennifer Marie Shemansky, University of Maryland, USA

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