Plant/Algal cell antibodies / DNA/RNA/cell cycle

product information

eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2,  elongation factor 1B-beta 2

recombinant eEF1B-beta protein from Arabidopsis thaliana  with no affinity tag,  Q9SI20

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha

AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2

collection of antibodies to DNA/RNA metabolism

to be added when available

application information

1: 1000 with standard ECL (WB)

25 | 30 kDa

Arabidopsis thaliana

dicots including: Brassica campestris. Pisum sativum, Ricinus communis,  monocots including: Hordeum vulgare, Oryza sativa, Zea mays

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available

application example

10 µg of total cell extract from  Arabidopsis thaliana (1), 5 µg of total cell extract from  Arabidopsis thaliana (2), 2 ng of recombinant eEF1b (3), 4 ng of recombinant eEF1b (4), 8 ng of recombinant eEF1b (5) were separated on  15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad).  Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).