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ExoS | Exoenzyme S

371 €

AS05 056 | clonality: polyclonal | host: hen | reactivity:  Pseudomonas aeruginosa

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Item No:
AS05 056

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product information
Background

Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.

Immunogen

amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.

Host Hen
Clonality Polyclonal
Clone
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 100 ĩl
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

Secondary antibodies

Additional information
application information
Recommended dilution

1: 5000 (WB)

Expected | apparent MW

48 kDa

Confirmed reactivity

Pseudomonas aeruginosa

Predicted reactivity

Pseudomonas aeruginosa only

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Anantharajah et al. (2017). Salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in Pseudomonas aeruginosa by distinct mechanisms. Antimicrob Agents Chemother. 2017 Apr 10. pii: AAC.02566-16. doi: 10.1128/AAC.02566-16. Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.


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