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Fer2 | ferrritin 2

345 €

AS06 196  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

PRODUCT INFORMATION IN PDF

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Item No:
AS06 196

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product information
Background

Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.

Immunogen

KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii 

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS06 126  Fer1 | ferritin 1(pre-apoferritin)

Collection of antibodies to Chlamydomonas proteins

Additional information
application information
Recommended dilution

1 : 1000

Expected | apparent MW

32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

a picture with a western blot application example can be found in the reference given below

Selected references Long et al. (2008) Genetics 179: 137-147


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