Ferritin (plant)

320 €

AS10 674 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

product information

background

Ferritin is the major non-toxic iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides.

immunogen

KLH-conjugated synthetic peptide derived from known plant ferritin sequences including Arabidopsis thaliana Q39101, At5g01600

antibody format

rabbit,

polyclonal

serum,

lyophilized

quantity

200 µl

- for reconstitution add 200 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS06 126 | anti-Fer1 | ferritin 1 (pre-apoferritin), algal antibody

additional information

to be added when available

application information
recommended dilution		1:3000 with standard ECL (WB)
expected \| apparent MW		28 \| 30 kDa
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		dicots: Phaseolus vulgaris, Solanum tuberosum, Ricinus communis, monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea mays, moss: Physcomitrella patens
not reactive in		Ostreococcus tauri
additional information		antibody is recognizing a chloroplastic form of plant ferritin
selected references		to be added when available, antibody released in June 2011

application example

10 µg of total protein from Arabidopsis thaliala whole seedlings, various cellular fractions, extracted with (see below*) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed four times for 5 min inPBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:15 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 180 seconds.

*Following is the protein extraction buffer composition that was used to extract the total protein from the whole seedlings:50mM Tris, 4% SDS, 1% PVPP , 5% Glycerol, 1mM PMSF, 2mM Pefabloc.

Courtesy of Anshika Jain and Erin Connolly, University of South Carolina, USA

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