Fibrinogen, labelled with fluorescein

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IMS09-038-335 clonality: polyclonal host: hen reactivity: human


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product information

Fibrinogen is the main protein of blood coagulation system. It is a large protein and it consists of two identical subunits that contain three polypeptide chains: alpha, beta and gamma. All chains are connected with each other by a number of disulfide bonds. Fibrinopeptides A (1 to 16 amino acids) and B (1 to 17 amino acids) are released by thrombin from the N-terminal parts of alpha and beta chains, respectively. In this way fibrinogen is converted into fibrin, which by means of polymerization forms a fibrin clot. Fibrinogen clotting underlies pathogenesis of MI, thromboembolism and thromboses of arteries and veins, since fibrin is the main substrate for thrombus formation. Fibrinogen activation is also involved in pathogenesis of inflammation, tumor growth and many other diseases. The normal fibrinogen concentration in plasma is about 3 mg/ml. The elevated level of fibrinogen in patient's blood is regarded as an independent risk factor for cardiovascular diseases. An increase in blood fibrinogen concentration was shown to be a strong predictor of coronary heart disease (Sonel et al. 2000; Rapold et al. 1989).


Purified, full length native fibrinogen Q9UE34

Host Hen
Clonality Polyclonal
Purity Affinity purified IgY
Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Quantity 100 ĩl (0.2mg/ml)

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Flow cytometry (FC)
Related products

IMS06-038-312 | Fibrinogen | biotinylated antibody

Additional information

The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation. Labelled with fluorescein. Affinity purified on human fibrinogen agarose.

application information
Recommended dilution

1: 10 (FC)

Expected | apparent MW

24 kDa

Confirmed reactivity

human, porcine, rat, rabbit

Predicted reactivity

bovine, mouse

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

the antibodies have been shown to react with activated human, porcine, rat and rabbit platelets

Selected references Terada et al. (2014). Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation on collagen via integrin αIIbβ3 activation. Transfusion. 2014 Feb 8. doi: 10.1111/trf.12566.
Schmidt et al. (2012). Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage. Clin. Microb. and Infection.

Application example


Flow cytometry: Suitable for detection of platelet activation by flow cytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704, Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated by centrifugation at room temperature. 5 ul platelet-rich plasma was added to polystyrene tubes containing 100 ul HEPES-buffer (137 mmol/L NaCl, 2.7 mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albumin, and 20 mmol/L HEPES, pH 7.4) and 10 ul FITC labelled chicken antibody. The samples were incubated for 10 minutes at room temperature and were then diluted and fixed with 1000 ul ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/L NaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing steps were used. The samples were analyzed utilising an Epics Profile XL-MCL cytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 platelets was carried out with the XL software (Coulter Electronics).

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