Fibrinogen, labelled with fluorescein
IMS09-038-335 clonality: polyclonal host: hen reactivity: human
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1: 10 (FC)
|Expected | apparent MW||
|Confirmed reactivity||human, porcine, rat, rabbit|
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
the antibodies have been shown to react with activated human, porcine, rat and rabbit platelets
|Selected references||Terada et al. (2014). Effects of riboflavin and ultraviolet light treatment on platelet thrombus formation on collagen via integrin αIIbβ3 activation. Transfusion. 2014 Feb 8. doi: 10.1111/trf.12566.
Schmidt et al. (2012). Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage. Clin. Microb. and Infection.
Flow cytometry: Suitable for detection of platelet activation by flow cytometry. Blood samples were collected in 5 mL sodium citrate tubes (367704, Becton Dickinson, Rutherford, NJ). Platelet-rich plasma was isolated by centrifugation at room temperature. 5 ul platelet-rich plasma was added to polystyrene tubes containing 100 ul HEPES-buffer (137 mmol/L NaCl, 2.7 mmol/L KCl, 1 mmol/L MgCl2 , 5.6 mmol/L glucose, 1 g/L bovine serum albumin, and 20 mmol/L HEPES, pH 7.4) and 10 ul FITC labelled chicken antibody. The samples were incubated for 10 minutes at room temperature and were then diluted and fixed with 1000 ul ice-cold PBS (0.02 mol/L Na2HPO4, 0.15 mol/L NaCl, 0.02% NaN3 , pH 7.2), containing 1 % p-formaldehyde. No washing steps were used. The samples were analyzed utilising an Epics Profile XL-MCL cytometer (Coulter Electronics, Hialeah, FL). Data processing from 5,000 platelets was carried out with the XL software (Coulter Electronics).
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